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Ph.D.
(Pharmacy)
DESIGN PHARMACOLOGICAL ELUCIDATION OF JUNIPERUS
COMMUNIS IN ANIMAL MODELS OF OBESITY AND
CHOLELITHIASIS
Ph.D. Scholar : Souravh Bais
Research Supervisor : Dr. Nilesh J. Patel

Regi. No.: 16146021015
Abstract :
Objective: Obesity is a metabolic disorder with limited FDA-approved drugs to treat and
standardized herbal plants have more potential and scope to treat the such metabolic
conditions. Gallstone disease or cholelithiasis is a disease caused by change in bile
secretions and cholesterol saturation to form crystals and blockage in the bile duct. The
current study focused on the Pharmacological elucidation of Juniperus Communis leaves
extract in various models of obesity and gallstone in mice model evaluate the anti-obesity
effect of Juniperus communis extract in various models of obesity.

Methodology: Various studies like microscopic analysis and macroscopic analysis were
carried out to identify the particular drug for the surety of raw material. Preliminary
phytochemical analysis, HPTLC study and method validation (Luteolin content)
performed to support the previous findings and to set up the phytochemical profile of the
plant. the extracts were evaluated for various in-vitro tests to access the potential of J.
Communis leaves against different enzymes and on the basis of their phytochemical
analysis and prescreening with enzymes the methanolic extract was selected to test in
adipogenesis in 3T3L1 mouse adipocytes and other enzyme inhibition assays like
Amylase, pancreatic Lipase, alpha-glucosidase and hydrogen peroxide assay were also
performed to determine the anti-diabetic activity. The cytotoxicity of the extract was
determined by the cell proliferation assay. An Acute oral toxicity study of methanolic
extract of Juniperus Communis was carried out according to OECD 425 guidelines in rats
and mice and 3 doses were selected, 150, 300, and 450 mg/kg to reveal it’s therapeutic
effect. High Fat Diet (HFD) induced obesity was developed in mice by administered HFD
for a period of 8 weeks. The anti-obesity and antiinflammatory effect of Methanolic
extract of Juniperus cummunis (MEJC) extract was accessed by treating at three doses
(150,300 & 450mg/kg, p.o.) mice for further 6 weeks. The various physiological and
biochemical parameters like weight gain, feed intake, serum lipid profile, serum
proinflammatory markers and histological changes in liver and visceral adipose tissue
were studied. In another model, mice received a one-time intra-peritoneal administration
of GTG (300mg/kg) at 4 weeks of age and observed for 8 weeks to induce obesity. The
anti-obesity and the anti-inflammatory effect were accessed by treating mice for further 6
weeks. The various physiological and biochemical parameters, serum lipid profile, and
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