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histological changes in liver and visceral adipose tissue were studied. In the Gallstone
model, Mice fed with the diet containing 1 % cholesterol and 0.5 % for six weeks resulted
in cholesterol super saturation in gallbladder bile, which promoted the formation of
cholesterol gallstones (CGS). In this study, the methanolic extract of J. Communis
(MEJC) were examined for antilithogenic their potential along with lithogenic (LG) diet for
seven weeks.

Results & Discussion: The MEJC extract showed the presence of important
phytoconstituents like luteolin, and Kaempferol and Ursodiol in the HPTLC Study followed
by the method validation of luteolin showed content of 156μg per 10mg of powder
extract. The extract was evaluated for its anti-obesity effect on adipocytes using 3T3L1
mouse adipocytes. J. Communis Extract showed the dose-dependent reduction in
triglycerides content in an assay. As per the results, it antagonizes the PPARγ through
Ser112 phosphorylation via MAPK/ERK activation. In differentiated adipocytes culture.
The extract treatment in differentiated adipocytes showed an increased (p =0.0169)
increased lipolysis as measured by the release of glycerol. J. Communis Extract does not
exhibit a cytotoxic effects on adipocytes. The MEJC extract at a high dose (450mg/kg,
p.o.) showed maximum effects to reduce obesity. From the present findings and
supporting literature, the study concludes that Orlistat, MEJC extract has a safe and
beneficial effect on reducing the obesity state of mice. J. Communis extracts reduced the
CGS incidence by 15–39%, the effect being a maximum in the methanol extract group.
The extract markedly reduced biliary cholesterol. The cholesterol: phospholipids ratio
which was 1.56 in the LG diet group was reduced to 0.92–0.97 in the J. Communis
extracts groups. Serum and liver cholesterol was decreased by feeding J. Communis
extracts to the LG diet.

Conclusion: From the present findings and supported literature, the study concludes that
Orlistat, MEJC extract has a safe and beneficial effect on reducing the obesity state of
mice. MEJC extract has a more potent effect in reducing weight and ameliorating the
weight-related medical complication. The above study also concludes that J. Communis
extract inhibits lipogenesis in adipocytes, antagonizes PPARγ and other lipogenic factors.
It also accelerates triglyceride mobilization from the fat cells or enhances lipolysis. The
other in-vitro enzyme inhibition was found with significant effect in treated groups
against cellular and oxidative damage caused by free radicals. J. Communis extracts
exerted antilithogenic influence by decreasing the cholesterol hyper-secretion into bile
and increasing the bile acid output thus decreasing the formation of lithogenic bile in
experimental mice. However, these findings should be further extrapolated through The
isolation of each active constituent and with other screening methods prior to its
application in clinical cases.

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