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Ph.D.
(Science)
ISOLATION, PRODUCTION AND OPTIMIZATION OF CELLULASE BY
MICROORGANISMS USING AGRICULTURAL WASTE

Ph.D. Scholar : Nikita Rajendrasinh Chavda
Research Supervisor : Dr. Pritiben H. Patel

Regi. No.: 18276481002
Abstract :
The development of appropriate procedures for the efficient treatment and utilization of
wastes containing cellulose as an inexpensive carbon source has grown to be of
substantial economic relevance. Cellulase enzyme, which is known to be produced by
microorganisms is responsible for degrading cellulose. Thus, isolation of microorganisms
producing cellulase was performed using soil sample and were identified as Bacillus
subtilis, Pseudomonas aeruginosa, Enterobacter cloacae using CMC medium. The
medium for fermentation was optimized for maximum cellulase to be produced by the
potential isolate using statistiscal approach. Various parameters like the time of
Incubation, temperature, pH, nitrogen sources, carbon sources, metal ions and NaCl were
considered for optimization. The culture condition was optimized and found to be 40°C at
pH 7 with maximum activity in the presence of ammonium sulphate and lactose as
nitrogen and carbon sources respectively. Amongst these isolates, the maximum
cellulase activity was shown by Enterobacter cloacae followed by Pseudomonas
aeruginosa and Bacillus subtilis by comparative study. The supplement for the medium
was various agricultural waste added as an alternate source of carbon to produce
cellulase. The medium with the presence of rice husk (1.76 IU/ml), followed by wheat
husk (1.51 IU/ml) and castor seed waste (0.65 IU/ml), had the highest cellulase activity.

Thus, this work aimed to compare the potential of all the above-mentioned isolates to use
agro-waste for production of cellulase at optimized parameters. The study's overall
findings identified the cellulolytic bacterial group that predominates in various habitat
types. Furthermore, the isolate's potential for usage in waste management and enzyme
synthesis was suggested by the use of agricultural waste material for the increased
cellulase production.
Key words: Agro waste; Bacteria; Cellulase; CMC; optimization; production.

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