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Ph.D.
(Pharmacy)
IN SITU GEL-BASEDMUCOSAL SITE SPECIFIC DRUG DELIVERY
SYSTEMS: FORMULATION AND EVALUATION

Ph.D. Scholar : Patel Kinjalben Rasiklal
Research Supervisor : Dr. Hitesh R. Patel

Regi. No.: 16146021012
Abstract :
The present work comprises three major chapters, which begin with an introductory part
and a literature survey.
In the present invention, mucoadhesive in situgel-based systems has emerged asa new
approach to drug delivery system.In situ,gel-forming systems are viscous liquids that
undergo to a gel phase upon exposure to physiological conditions. The formation of the
gel depends on the factors such as temperature fluctuation, pH changes, and ion
exposure from which the drug is released continuously and in a controlled
manner.Controlled release of drugs offers the benefit of reduced administration
frequency and improved patient compliance. The dose of the drug is reduced which is
why the toxicity, is compared to conventional treatment.

In the present research work, an attempt has been made to formulate metronidazole and
clotrimazole in situ gel for the effective management of periodontitis, and vaginal
candidiasis, & otomycosis with local delivery for a controlled release. For this study,
various concentrations of Poloxamer 407, Poloxamer 188, carbopol 974, gellan gum,
HPMC E50, Natrosol 250, and Klucel HF polymers were used. The formulated in situ gel
was evaluated for clarity, in vitro gelling capacity, gelation time, pH, viscosity, gelation
temperature, % drug content, spreadability, in vitro drug release, and in vitro antimicrobial
study. 32 full factorial designs and desirability functions were applied. All the formulations
were developed using different concentrations of the above polymers. The developed
formulations showed satisfactory results for different evaluation parameters. We had
considered the best formulation based on desirability and overlay plot.

Key words: Mucoadhesive in situ gel, metronidazole, clotrimazole, periodontitis, vaginal
candidiasis, otomycosis,Poloxamer 407, Poloxamer 188, carbopol 974, gellan gum, HPMC
E50, Natrosol 250, Klucel HF, factorial design, desirability

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